Journal: Journal of Translational Medicine

Article Title: Molecular target based combinational therapeutic approaches in thyroid cancer

doi: 10.1186/1479-5876-10-81

Figure Lengend Snippet: Effect of estrogen on the in vitro VEGF secretion by thyroid cancer cells. B-CPAP and ML-1 thyroid cancer cells were seeded at a density of 5X10 5 cells per well in six-well culture dishes and allowed to adhere overnight. Thyroid cancer cells were then switched to serum free medium and incubated with ± 10 -8 M E 2 ± 10 -6 M fulvestrant or left untreated for 24 hours. The conditioned medium was harvested and ( A ) B-CPAP and ( B ) ML-1 conditioned medium was assayed for VEGF secretion using VEGF ELISA kit. The asterisk denotes statistically significant differences ( p < 0.05) between experimental and untreated samples determined using a paired Student’s t - test. The standard deviation represents n = 3 for each sample.

Article Snippet: The RayBio® Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit (RayBiotech, Inc., Norcross GA) was used for quantitative measurement of VEGF secreted in the thyroid cancer cell conditioned medium.

Techniques: In Vitro, Incubation, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Journal of Translational Medicine

Article Title: Molecular target based combinational therapeutic approaches in thyroid cancer

doi: 10.1186/1479-5876-10-81

Figure Lengend Snippet: DIM targets VEGF secretion of thyroid cancer cells. B-CPAP and ML-1 thyroid cancer cells were seeded at a density of 5×10 5 cells per well in six-well culture dishes and allowed to adhere overnight. Thyroid cancer cells were then switched to serum free medium and incubated with ± 10 -8 M E 2 ± 10 -6 M fulvestrant or left untreated for 24 hours. The conditioned medium was harvested and ( A ) ML-1 and ( B ) B-CPAP VEGF was assayed using VEGF ELISA kit. The asterisk denotes statistically significant differences ( p < 0.05) between the treatment and untreated samples determined using a one way ANOVA test. The standard deviation represents n = 3 for each sample.

Article Snippet: The RayBio® Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit (RayBiotech, Inc., Norcross GA) was used for quantitative measurement of VEGF secreted in the thyroid cancer cell conditioned medium.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Cell Death & Disease

Article Title: Cannabidiol promotes apoptosis via regulation of XIAP/Smac in gastric cancer

doi: 10.1038/s41419-019-2001-7

Figure Lengend Snippet: a , b OCR in CBD-treated or -untreated cells was measured using an XF24 extracellular flux analyzer by adding oligomycin, CCCP, and rotenone ( a ). Basal respiration (left) and ATP production (right) were quantified using the obtained OCR ( b ). * P < 0.05 and ** P < 0.01. c CBD-treated or untreated cells were incubated with JC-1 at 37 °C for 30 min. MMP of stained cells was determined by immunofluorescence analysis. d Cells were treated with 4 μM of CBD for 24 h. Treated cells were stained with TMRE dye at 37 °C for 30 min. The representative images were captured using a confocal microscope. e Western blotting analysis of mitochondria electron transport proteins in CBD treatment. f Mitochondria was isolated from CBD-treated or untreated AGS cells. Smac expression in the cytosolic and mitochondrial fractions was analyzed by western blotting. g Smac release was measured by using a Smac ELISA kit. *** P < 0.001

Article Snippet: Human Smac ELISA kit was purchased from RayBioTech (GA, USA).

Techniques: Incubation, Staining, Immunofluorescence, Microscopy, Western Blot, Isolation, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Virulence

Article Title: Hemagglutinin protein of Peste des Petits Ruminants virus (PPRV) activates the innate immune response via Toll-like receptor 2 signaling

doi: 10.1080/21505594.2021.1882246

Figure Lengend Snippet: DCs produce pro-inflammatory cytokines and chemokines in response to H protein activation. Monocyte-derived DCs were stimulated with Pam3 (1 μg/ml), Pam 2 (5 μg/ml), increasing amounts of PPRV H protein (10, 5 and ng/ml) and increasing amounts of inactivated PPRV ICV’89 (1:10 and 1:100 dilutions). (a) At 24 h post-stimulation RNA was isolated, reverse transcribed, and real-time PCR carried out using specific primers for each cytokine or chemokine. Values are expressed as fold-change of mRNA values related to Mock cells. (b) At 24 h post-stimulation IL-12 secretion into the DC culture supernatants was determined by ELISA

Article Snippet: Cell supernatants were collected and analyzed by IL-12 content by ovine IL-12 p70 ELISA kit (RayBiotech), following manufacturer’s instruction.

Techniques: Activation Assay, Derivative Assay, Isolation, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Critical Care Explorations

Article Title: Complement Activation in Patients With Heat-Related Illnesses: Soluble CD59 Is a Novel Biomarker Indicating Severity of Heat-Related Illnesses

doi: 10.1097/CCE.0000000000000678

Figure Lengend Snippet: Serial changes in complement activation in patients with heat-related illnesses. A – E , Complement component 3a (C3a), complement component 5a (C5a), Ba fragment of complement factor B (Ba), soluble complement C5b-9, and soluble CD59 (sCD59) significantly increased in patients with heat-related illnesses on day 0 compared with those in the healthy controls (HCs). Data shown are median (interquartile range), and the groups were compared using Kruskal-Wallis test with Dunn post hoc test. F , Although difference in Factor H among the four groups, Dunn post hoc test did not show differences between the HCs and patients with heat-related illnesses on day 0. Data in blue and red are from patients who died.

Article Snippet: The plasma level of CD59 (sCD59) was measured using the RayBio Human CD59 Enzyme-Linked Immunosorbent Assay kit (ELH-CD59 RayBiotech, Norcross, GA).

Techniques: Activation Assay

Journal: Critical Care Explorations

Article Title: Complement Activation in Patients With Heat-Related Illnesses: Soluble CD59 Is a Novel Biomarker Indicating Severity of Heat-Related Illnesses

doi: 10.1097/CCE.0000000000000678

Figure Lengend Snippet: Complement system is activated in patients with severe heat-related illnesses. A – D , Complement component 3a (C3a), complement component 5a (C5a), Ba fragment of complement factor B (Ba), and soluble complement C5b-9 significantly increased in patients with stage II and stage III heat-related illnesses on day 0 compared with those in the healthy controls (HCs). Data shown are median (interquartile range), and the groups were compared using Kruskal-Wallis test with Dunn post hoc test. E , Stage III patients with heat-related illnesses showed a significant difference in the soluble CD59 (sCD59) level compared with the HCs, stage I patients, and stage II patients. F , Factor H did not demonstrate a significant difference between the HCs and patients with heat-related illnesses. Data in blue and red are from patients who died.

Article Snippet: The plasma level of CD59 (sCD59) was measured using the RayBio Human CD59 Enzyme-Linked Immunosorbent Assay kit (ELH-CD59 RayBiotech, Norcross, GA).

Techniques:

Journal: Critical Care Explorations

Article Title: Complement Activation in Patients With Heat-Related Illnesses: Soluble CD59 Is a Novel Biomarker Indicating Severity of Heat-Related Illnesses

doi: 10.1097/CCE.0000000000000678

Figure Lengend Snippet: Correlation of soluble CD59 (sCD59) with Acute Physiology and Chronic Health Evaluation II (APACHE II) score ( A ), Sequential Organ Failure Assessment (SOFA) score ( B ), stage ( C ), and Japanese Association for Acute Medicine disseminated intravascular coagulation (DIC) score ( D ). A – C , Spearman rank correlation coefficient, p < 0.0001 ( n = 32). The data points shown ( A , 44; B , 47) are of samples from days 0, 3–4, and 5–6 following a diagnosis of heat-related illnesses, and the 32 data points ( C ) consist of samples from day 0. D , Spearman rank correlation coefficient, p = 0.0065 ( n = 32). The 47 data points shown are of samples from days 0, 3–4, and 5–6 following a diagnosis of heat-related illnesses.

Article Snippet: The plasma level of CD59 (sCD59) was measured using the RayBio Human CD59 Enzyme-Linked Immunosorbent Assay kit (ELH-CD59 RayBiotech, Norcross, GA).

Techniques: Coagulation

Journal: Critical Care Explorations

Article Title: Complement Activation in Patients With Heat-Related Illnesses: Soluble CD59 Is a Novel Biomarker Indicating Severity of Heat-Related Illnesses

doi: 10.1097/CCE.0000000000000678

Figure Lengend Snippet: Correlation of soluble CD59 (sCD59) with fibrin-degradation product. A and B , Spearman rank correlation coefficient, not significant ( n = 32). The 46 data points shown consist of samples from days 0, 3–4, and 5–6 following a diagnosis of heat-related illnesses. C , Spearman rank correlation coefficient, p = 0.0107 ( n = 32). The 39 data points shown consist of samples from days 0, 3–4, and 5–6 following a diagnosis of heat-related illnesses. D , Spearman rank correlation coefficient, not significant ( n = 32). The 47 data points shown are of samples from days 0, 3–4, and 5–6 following a diagnosis of heat-related illnesses. APTT = activated partial thromboplastin time, FDP = fibrin degradation product, Plt = platelet, PT = prothrombin time.

Article Snippet: The plasma level of CD59 (sCD59) was measured using the RayBio Human CD59 Enzyme-Linked Immunosorbent Assay kit (ELH-CD59 RayBiotech, Norcross, GA).

Techniques: